FREE FAT IN MILK AND CHEESE PRODUCTS: INFLUENCE ON QUALITY

The theoretical and practical aspects of the formation of free fat in milk, cheese and cheese products with vegetable fats are considered. The amount of free fat in milk depends on the integrity of the fat globules membranes, which are affected by: ineffective emulsification of fat during the synthesis of milk in the cow’s udder (authentic fat) and physic-mechanical factors in the milk processing after milking (destabilized fat). Free fat, primarily subjected to oxidative processes and lipolysis, reduces the quality and ability to store milk. For cheese products, the presence of free fat, available for lipolysis and oxidation, is a prerequisite for obtaining high-quality products. Characteristics of the production of cheese products with vegetable fats, providing for the preliminary emulsification of vegetable fat in skim milk, contribute to the formation of denser membranes on the surface of fat globules, consisting mainly of casein micelles and whey proteins. This is the reason for the formation of a more closed structure of the fat phase with low availability of fat for enzymes and oxidizing agents. The low availability of fat in the structure of cheese products with vegetable fats is one of the factors that worsen their organoleptic characteristics. In order to increase the amount of available fat in the production of cheese products with vegetable fats, it is advisable to use additional enzyme preparations or cultures of microorganisms that activate proteolysis and lipolysis.The theoretical and practical aspects of the formation of free fat in milk, cheese and cheese products with vegetable fats are considered. The amount of free fat in milk depends on the integrity of the fat globules membranes, which are affected by: ineffective emulsification of fat during the synthesis of milk in the cow’s udder (authentic fat) and physic-mechanical factors in the milk processing after milking (destabilized fat). Free fat, primarily subjected to oxidative processes and lipolysis, reduces the quality and ability to store milk. For cheese products, the presence of free fat, available for lipolysis and oxidation, is a prerequisite for obtaining high-quality products. Characteristics of the production of cheese products with vegetable fats, providing for the preliminary emulsification of vegetable fat in skim milk, contribute to the formation of denser membranes on the surface of fat globules, consisting mainly of casein micelles and whey proteins. This is the reason for the formation of a more closed structure of the fat phase with low availability of fat for enzymes and oxidizing agents. The low availability of fat in the structure of cheese products with vegetable fats is one of the factors that worsen their organoleptic characteristics. In order to increase the amount of available fat in the production of cheese products with vegetable fats, it is advisable to use additional enzyme preparations or cultures of microorganisms that activate proteolysis and lipolysis

CD56 and Tim-3 molecule expression in different monocyte subsets in physiological pregnancy

Monocytes play an important role in the systemic immune defense against pathogens and maintaining physiological pregnancy. During pregnancy peripheral monocytes migrate into the decidua and form the pool of decidual macrophages which participate in the formation and development of placental tissues. The population of peripheral blood monocytes is phenotypically and functionally heterogeneous. In humans, there are different monocyte subsets depending on the expression of CD14 and CD16. CD56-positive monocytes are found in healthy women. Their number is positively correlated with body mass index, body fat. Tim-3 (T cell Ig and mucin domain-containing protein 3) expression is observed in peripheral monocytes during pregnancy. It is known that peripheral monocyte functions effectively change at pregnancy to form the immune tolerance at the maternal-fetal interface and the systemic immune defense against pathogens. However, the monocyte phenotype shift during pregnancy remain poorly understood. Therefore, the aim of the study was to evaluate the CD56 and Tim-3 expressions in monocyte subsets in human pregnancy. Peripheral blood mononuclear cells were isolated from peripheral blood of pregnant women (gestational age 29 weeks (28-31) by density gradient centrifugation and analyzed by flow cytometry. Peripheral blood of healthy non-pregnant fertile women (in follicular phase of the menstrual cycle) aged 21-29 years was studied as control. Pregnant women had a lower percentage of classical CD14hi/CD16- monocytes in comparison with non-pregnant. The percentages of intermediate (CD14hi/CD16+) and non-classical (CD14low/CD16+) monocytes did not change. The CD56 molecule expression was observed in all monocyte subsets in pregnant and non-pregnant women. Pregnant women had a higher percentage of CD56-positive classical (CD14hiCD16-) and non-classical (CD14lowCD16+) monocytes than non-pregnant. The percentage of CD56-positive intermediate (CD14hiCD16+) monocytes did not change. The percentages of double-positive CD56+Tim-3+ classical (CD14hiCD16-) and non-classical (CD14lowCD16+) monocytes were increased in pregnant women. The numbers of double-positive CD56+Tim-3+intermediate (CD14hiCD16+) monocytes did not change. Thus, the CD56 and Tim-3 expressions in different monocyte subsets were changed in human pregnancy

Phosphorylation of histone H3Ser10 in plant cell division

Histones, the major protein components of chromatin, undergo post-translational modifications, which particularly affect the structural and functional organization of chromosomes. The most common post-translational modifications are phosphorylation, methylation, acetylation and ubiquitination. Histone phosphorylation occurs mainly at N-terminal tails of serines (Ser) and threonines (Thr), and coordinates various processes of mitotic and meiotic division. It has been shown that this type of modification is required for activation of transcription, DNA damage repair, recombination and also for chromosome condensation and segregation. Histone H3 is characterised by the presence of a large number of modification sites among the four core histones. In plants, phosphorylation of histone H3 at serine positions 10 and 28 and at threonine positions 3, 11, 32 and 133 is the most well studied. This review contains the most complete data on the spatial and temporal distribution of H3 phosphorylation of serine at position 10 (phH3Ser10) in mitosis and meiosis in different plant species. Most species are characterised by phosphorylation of the centromeric region in mitosis and second meiotic division, and by phosphorylation throughout the chromosomes in the first meiotic division. However, there are exceptions to the phH3Ser10 distribution in mosses and cestrum, as well as in species with holocentric chromosomes. There are contradictory data on the phH3Ser10 distribution in mitosis and meiosis in the same species. The functional significance of phH3Ser10 in cell division in plants is associated with the activity of the centromere, centromere cohesion and sister chromatid and chromosome segregation. We discuss the participation of currently known candidate kinases and phosphatases in the dynamics of H3Ser10 phosphorylation. The review provides an overview of the role of phH3Ser10 modification in the chromosome division and segregation in mitosis and meiosis

Evaluation of the microstructure and mechanical properties of a new modified cast and laser-melted AA7075 alloy

The mechanical properties and microstructure of as-cast and homogenized AA7075 were investigated. This alloy was modified by adding transition elements 0.3%Sc + 0.5%Zr, 1%Ti + 0.2%B, and 1%Fe + 1%Ni for use in additive manufacturing applications. After adding Ti + B and Sc + Zr, the structure became uniform and finer with the formation of the Al3(Sc, Zr) and TiB2 phases. Coarse structures were obtained with the formation of an extremely unfavorable morphology, close to a needle-like structure when Fe + Ni was added. The mechanical properties of the modified alloys were increased compared to those of the standard alloy, where the best ultimate tensile strength (UTS) and yield strength (YS) were obtained in the AA7075-TiB alloy compared to the standard alloy in as-cast and homogenized conditions, and the highest hardness value was provided by Fe + Ni additives. The effect of the laser melting process on the microstructure and mechanical properties was investigated. Single laser melts were performed on these alloys using 330 V and a scanning speed of 8 mm/s. During the laser melting, the liquation of the alloying elements occurred due to non-equilibrium solidification. A change in the microstructures was observed within the melt zone and heat-affected zone (HAZ). The hardness of the laser-melted zone (LMZ) after adding the modification elements was increased in comparison with that of the standard alloy. Corrosion testing was performed using a solution of 100 mL distilled water, 3.1 g NaCl, and 1 mL HCl over 5, 10, and 30 min and 1 and 2 h. The corrosion resistance of the alloy modified with FeNi was low because of the non-uniform elemental distribution along the LMZ, but in the case of modification with ScZr and TiB, the corrosion resistance was better compared to that of the standard alloy. © 2019 by the authors.Ministry of Science and Higher Education of the Russian FederationThe author (Asmaa M. Khalil) gratefully acknowledges financial support from the Ministry of Science and Higher Education of the Russian Federation in the framework of Increase Competitiveness Program of MISiS (Support project for young research engineers)

The role of iron metabolism indicators in predicting gestational diabetes mellitus

Iron (Fe) deficiency and hyperglycaemia are both widely found throughout the world among pregnant women. According to the latest data from the American Diabetes Association (ADA), the prevalence of gestational diabetes mellitus (GDM) has increased and reaches 15–20%. Nowadays, there is growing evidence that a link between Fe metabolism and glucose homeostasis exists. The study of changes in the indicators of Fe metabolism in the serum of pregnant women with glucose intolerance and GDM plays an important role in expanding the understanding of the pathogenesis of these conditions. The hypothesis that excess Fe increases the risk of developing GDM has prompted us to review and evaluate the potential relationship between elevated Fe levels and the risk of developing GDM. The aim is to integrate all available data on the correlation between GDM and Fe status in the body. It is increasingly being recognised that excess Fe accumulation in the body is associated with an increased risk of diabetes. There is no available data on the relationship between serum ferritin and GDM in the Russian population, while early identification of the risk of GDM development will be of great importance for its related health effects and prevention. The role of Fe status as a GDM biomarker in high-risk populations is of interest, both for prognostic and diagnostic measures, and for therapeutic interventions. For a better understanding of whether an excess of Fe increases the risk of developing GDM, studies are needed to reveal the role of Fe in the mechanisms of GDM development

Response of cardiorespiratory system during maximal exercise cycle ergometer test in women cross-country skiers with different skills levels

Objective: to estimate the functional features of the cardiorespiratory system of female cross-country skiers with different skills levels at the maximal bicycle ergometer test.Material and methods: 70 female cross-country skiers were divided into three groups. The first group included 21 cross-country skiers with first adult sporting rank, the second — 25 candidates in master of sports, the third group — 24 sport masters in cross-country skiing. Maximal exercise tests were used to determine cardiorespiratory fitness via spiroergometric system «Oxycon Pro» (Jaeger, Hoechberg, Germany).Results: The most notable differences were observed between the first-class and the masters of the sports groups. In the first-class group the heart rate during the maximal exercise test was, on average, 11 beats/min higher than in the master of sports group (p<0.05), and the oxygen consumption at the anaerobic threshold was, on average, lower by 7.7 ml/min/kg (p < 0.05) when comparing the same groups.Conclusion: The influence of skills levels on physiological parameters at maximal bicycle ergometer test was shown. The capacity in the first group occurs due to cardiovascular system tension, and the same in the group high qualification skiers happen due to the mechanisms of oxygen transport development

Galectin-9 influences the Tim-3 molecule expression in natural killer different subpopulations

Galectin-9 is a b-galactoside binding lectin with expressed immunoregulatory activity. During pregnancy galectin-9 is produced by trophoblast cells and regulates the function of natural killer (NK) cells at the maternal-fetal interface via binding to Tim-3 (T-cell Ig and mucin domain-containing protein 3) molecules. Natural killer (NK) lymphocytes belong to the innate lymphoid cells, which have a cytotoxic effect on target cells and are capable of producing a large number of regulatory factors (cytokines, chemokines). Decidual NK have a tolerant phenotype and play a leading role in the regulation of invasive trophoblast growth and provide peripheral immune tolerance in the area of uteroplacental contact. Peripheral NK cells express Tim-3 molecules. Galectin-9 concentration is increased in peripheral blood during physiologic pregnancy. At pregnancy phenotype and functions of peripheral NK cells are changed to maintain the maternal–fetal immune tolerance. Peripheral NK cells migrate to the maternal-fetal interface and are transformed into a decidual NK-like phenotype cells. Galectin-9 concentration is decreased in women with a  complicated  pregnancy and miscarriage. However the galectin-9 effects on different NK cell subpopulations of peripheral blood are not investigated. Therefore, we studied the galectin-9 influence on phenotype transformation and Tim-3 expression of NK cells isolated from peripheral blood of healthy non-pregnant fertile women. CD56+NK cells were obtained by immunomagnetic separation and cultivated in vitro during 72 hours with cytokines (IL-2 and IL-15). Galectin-9 (5 ng/mL) and anti-Tim-3 (10 mg) antibodies were added to the NK cultures. Galectin-9 concentration is corresponded to its level during first trimester of physiologic pregnancy. The number of regulatory NK (CD16-CD56bright), cytotoxic NK (CD16+CD56dim/-) cells and Tim-3 expression on different NK subpopulations were assessed by flow cytometry. It was found that Tim-3 was expressed on all subpopulations of peripheral blood NK cells (CD16-CD56brightNK, CD16+CD56dimNK, CD16+CD56-NK). Incubation with galectin-9 increased the expression of Tim-3 on regulatory CD16-CD56brightNK cells and did not change on cytotoxic CD16+CD56dim/-NK cells. Galectin-9 reduced the percentage of cytotoxic CD16+CD56dimNK in culture, but did not influence the number of regulatory CD16-CD56bright NK and cytotoxic CD16+CD56-NK cells. Thus, galectin-9 regulates Tim-3 molecule expression and NK cell subpopulation distributions in vitro culture

Influence of Adding Modifying Elements and Homogenization Annealing on Laser Melting Process of the Modified Alznmgcu with 4%si Alloys

AlZnMgCu, the high-strength aluminum alloy, is unsuitable for laser melting applications due to its high hot cracking sensitivity and large solidification temperature range. Adapting this alloy for laser melting processing is a high-demand research issue for extending its use. Thus, this paper investigates the effect of adding 4%Si, 4%Si-Sc + Zr, 4%Si-Ti + B, and homogenization annealing on the laser melting process (LMP) of AlZnMgCu alloy. Homogenization annealing at 500◦ C for 6.5 h was selected to dissolve most of the low melting temperature phases into the grain matrix and perform stable alloys for the LMP. The pulsed laser melting process (PLM) was performed on the as-casted and the homogenized samples. The microstructures of the as-casted, the homogenized alloys, and after the LMP were evaluated. In addition, the hardness of the base metal (BM) and laser melted zone (LMZ) were measured. The results revealed that the microstructure was enhanced and refined in the as-cast state by adding the modifiers due to the increasing nucleation potency of solidification sites and the formation of primary Al3 (Ti, Zr, Sc) phases. The average grain size was decreased by 15.6 times when adding 4%Si + 0.4%Zr + 0.29%Sc, while it decreased by 10.2 times when adding 4%Si + 1%Ti + 0.2%B. The LMZ of the as-casted samples exhibited a non-uniform distribution of the grains and the elements after the LMP. This was attributed to the evaporation of Zn, Mg during the high laser power process besides the non-uniform distribution of elements and phases in samples during casting. After the laser treating of the homogenized samples with 4%Si-Sc + Zr, uniform columnar grains were formed in the direction of the laser. The presence of Ti and B changed the crystallization nature, resulting in the LMZ with very fine and equiaxed grains due to forming many nucleation centers during solidification. The hardness values have positively increased due to Si addition and adding a combination of Ti + B and Sc + Zr. The maximum hardness was 153.9 ± 5 HV achieved in the LMZ of the homogenized samples of 4%Si + 1%Ti + 0.2%B. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.Funding: This research was partially funded by Russian Foundation for Basic Research (RFBR) with project number 19-38-60037

Первые результаты 6-летнего динамического наблюдения пациента с ранним псориатическим артритом, леченного в рамках стратегии «Лечение до достижения цели»

The paper characterizes the basic principles of a treat-to-target (T2T) strategy for spondyloarthritis, including psoriatic arthritis (PsA). The data from observational cohort studies suggest that inadequate therapy for PsA increases the risk of structural progression. The results, obtained in the international randomized controlled Tight Control of Psoriatic Arthritis (TICOPA) trial and the Russian open-label observational REMARCA study, have justified the necessity of using the T2T strategy for early-stage PsA. The authors have analyzed their own results of a 6-year follow-up study of a patient with early PsA, in whom the T2T strategy was used.В статье приводится характеристика основных принципов стратегии «Лечение до достижения цели» («Treat to target», T2T) при спондилоартритах, включая псориатический артрит (ПсА). Данные наблюдательных когорт свидетельствуют о том, что отсутствие адекватной терапии ПсА увеличивается риск структурного прогрессирования. На основании результатов, полученных в международном рандомизированном контролируемом исследовании TICOPA, а также Российском открытом наблюдательном исследовании РЕМАРКА, обоснована необходимость использования стратегии Т2Т на ранней стадии ПсА. Проанализированы собственные результаты 6-летнего наблюдения за пациентом с ранним ПсА, при лечении которого была применена стратегия Т2Т

ПЕРСПЕКТИВИ ЗАПРОВАДЖЕННЯ ЛІКАРСЬКОЇ РЕЗИДЕНТУРИ У ПЕДІАТРІЇ

The aim of the work – to highlight the importance of medical residency in the training of pediatricians.The main body. Medical residency should be conducted at medical universities, academies, institutes, and should become a form of specialization of doctors in certain medical specialties (after completion of internship in basic specialties), to be conducted exclusively at the appropriate clinical departments in order to obtain the qualification of a specialist doctor in accordance with the list of medical specialties approved by the Ministry of Health of Ukraine.The main task of the pediatric medical residency is deepening of the level of practical training of children’s doctors, their mastery of a certain level of professional abilities, practical skills, duties and competences within the specific medical specialization, necessary for independent medical activity in a particular pediatric specialty.Conclusion. The introduction of a medical residency in postgraduate medical education is an urgent requirement of time, is to be one of the urgent needs of the healthcare sector being reformed, and will contribute to improving the quality of postgraduate training of pediatricians and, accordingly, the preservation of children’s health.Мета роботи – висвітлити значення лікарської резидентури у підготовці лікарів-педіатрів.Основна частина. Лікарська резидентура має проводитись у медичних університетах, академіях, інститутах та стати формою спеціалізації лікарів-спеціалістів за певними лікарськими спеціальностями (після закінчення інтернатури за базовими спеціаль­ностями), що буде проводитись виключно на відповідних клінічних кафедрах для отримання кваліфікації лікаря-спеціаліста згідно з переліком лікарських спеціальностей, затвердженим Міністерством охорони здоров’я України.Основним завданням педіатричної лікарської резидентури є поглиблення рівня практичної підготовки дитячих лікарів, оволо­діння ними певного рівня професійних умінь, практичних навичок, обов’язків та компетенцій у межах конкретної лікарської спеціалізації, необхідних для самостійної лікарської діяльності за певною педіатричною спеціальністю.Висновок. Запровадження лікарської резидентури у післядипломній медичній освіті є нагальною вимогою часу, відповідатиме нагальним потребам галузі охорони здоров’я, що реформується, та буде сприяти підвищенню якості післядипломної підготовки лікарів-педіатрів і, відповідно, збереженню здоров’я дітей